Payment extra fat try mentioned pursuing the an overnight timely having fun with air-displacement plethysmography (BOD POD Express; Lifestyle Aspect, Concord, California, USA)
Sides and you can cool circumferences was indeed measured on participants within the an excellent informal status posture. Waist width was removed during the mid-part within prior premium iliac spine plus the straight down edge of the rib crate, and hip width within quantity of the greater amount of trochanter. All the proportions were made regarding the research period because of the a single observer (Elizabeth. L. Lim).
Logical tips
Plasma glucose concentration was measured by the glucose oxidase method (YSI glucose analyser; Yellow Springs, OH, USA), plasma insulin and C-peptide concentrations by ELISA (Dako, Ely, UK), plasma triacylglycerol by lipase with released glycerol measured by a Roche Cobas centrifugal analyser using a colorimetric assay (ABX Diagnostics, Montpellier, France) and HbA1c by a Biorad HPLC (TOSOH Corporation, Tokyo, top volledig gratis dating sites Japan). 2 H atom percent excess in plasma glucose was determined using a Thermo ‘Voyager’ single quadrupole mass spectrometer with Thermo ‘Trace’ gas chromatograph (Thermo Scientific, Waltham, MA, USA).
Analytical steps
Statistical analyses were performed using SPSS 17.0 software (SPSS Inc., Chicago, IL, USA). Data are presented as mean ± SE. Statistical comparisons between diabetes and control groups were performed using the Student’s t test, while within-group differences were determined using a paired t test. Changes of sequential data within experiments were evaluated by repeated measures one-way ANOVA with post hoc Bonferroni testing where appropriate. (tovább…)